LC-tandem mass spectrometry as a screening tool for multiple detection of allergenic ingredients in complex foods

Authors

  • Linda Monaci CNR-ISPA
  • Rosa Pilolli CNR-ISPA
  • Elisabetta De Angelis
  • Rossella Carone
  • Michelangelo Pascale

DOI:

https://doi.org/10.21014/acta_imeko.v5i1.278

Abstract

In the present investigation, an LC-MS/MS method for sensitive multiplex detection of five allergenic ingredients in a processed food matrix is presented. Cookie was chosen as complex food model and was incurred with egg, milk, soy, hazelnuts and peanuts before baking. Extraction, purification and pre-concentration protocols were applied to ground cookie basing on protocols described elsewhere. Specific instrumental features of a dual cell linear ion trap MS instrument were exploited to identify suitable peptide markers for each allergen and to deliver a sensitive multiplex SRM-based method for the simultaneous detection of common allergenic ingredients which might contaminate such a commodity.

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Published

2016-04-29

Issue

Section

Research Papers