LC-tandem mass spectrometry as a screening tool for multiple detection of allergenic ingredients in complex foods

Authors

  • Linda Monaci CNR-ISPA
  • Rosa Pilolli CNR-ISPA
  • Elisabetta De Angelis
  • Rossella Carone
  • Michelangelo Pascale

DOI:

https://doi.org/10.21014/acta_imeko.v5i1.278

Abstract

In the present investigation, an LC-MS/MS method for sensitive multiplex detection of five allergenic ingredients in a processed food matrix is presented. Cookie was chosen as complex food model and was incurred with egg, milk, soy, hazelnuts and peanuts before baking. Extraction, purification and pre-concentration protocols were applied to ground cookie basing on protocols described elsewhere. Specific instrumental features of a dual cell linear ion trap MS instrument were exploited to identify suitable peptide markers for each allergen and to deliver a sensitive multiplex SRM-based method for the simultaneous detection of common allergenic ingredients which might contaminate such a commodity.

Downloads

Published

2016-04-29

Issue

Vol. 5 No. 1 (2016)

Section

Research Papers

License

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under the CC BY 4.0, Creative Commons Attribution 4.0 International License.

Users are free to

  • share, i.e. copy and redistribute the material in any medium or format for any purpose, even commercially;
  • adapt, i.e. remix, transform, and build upon the material for any purpose, even commercially.

At the same time, the user must give appropriate credit, provide a link to the license, and indicate if changes were made.
Additional information about the license can be found at: https://creativecommons.org/licenses/by/4.0/.

Authors are

  • able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).