Determination of sulfonamides in muscle: a metrological tool for food safety


  • Irene Diamanti Istituto Zooprofilattico Sperimentale dell Umbria e delle Marche Togo Rosati
  • Roberta Galarini Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Perugia
  • Raffaella Branciari Departement of Veterinary Medicine, University of Perugia, Perugia, Italy
  • Giorgio Saluti Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “Giuseppe Caporale”, Teramo
  • Laura Fioroni Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Perugia



sulfonamides, residues, LC-HRMS, multiclass method, food control, measurements


Sulfonamides represent a wide class of synthetic drugs commonly used in veterinary therapy for the treatment of several bacterial and protozoan infections in cattle, swine and poultry. The use of these drugs in farming can lead to the possibility of having their residues in animal products intended for human consumption. Consequently, to ensure high consumer protection, for sulfonamides European Union (EU) set a Maximum Residue Limit (MRL) equal to 100 µg/kg, either as a single molecule or as a sum of all detected compounds within the class). Official laboratories are directly involved in the execution or residue plans by developing, validating and then applying analytical methods for the measurement of drug residues. Accordingly, official laboratories should update their procedures following the evolution of required drugs and MRLs. A multiclass method previously developed and validated for the determination in animal muscle of ten classes of antibiotics was adjusted to comply with the current European requirements which establish the minimum set of sulfonamides to be determined. Therefore, eight new sulfonamides were added assessing method performance characteristics according to European Regulation (EU) 808/2021.






Research Papers