A Principal Componet Analysis to detect cancer cell line aggressiveness

Authors

  • Livio D'Alvia La Sapienza University of Rome, Italy http://orcid.org/0000-0003-2952-5655
  • Serena Carraro La Sapienza University of Rome, Italy
  • Barbara Peruzzi Bambino Gesù Children's Hospital, Italy
  • Enrica Urciuoli Bambino Gesù Children's Hospital, Italy
  • Ludovica Apa La Sapienza University of Rome, Italy
  • Emanuele Rizzuto La Sapienza University of Rome, Italy

DOI:

https://doi.org/10.21014/actaimeko.v12i2.1136

Keywords:

measurement of dielectric properties, biosensor, non-invasive measurements, cancer cell lines, cancer aggressiveness, osteosarcoma, breast cancer, PCA, Principal Component

Abstract

In this paper, we propose the use of Principal Component Analysis (PCA) as a new post-processing method for the detection of breast and bone cancer cell lines cultured in vitro using a microwave biosensor. MDA-MB-231 and MCF7 breast cancer cell lines and SaOS-2 and 143B osteosarcoma cell lines were characterized using a circular patch resonator in the 1 MHz – 3 GHz frequency range. The return loss of each cancer cell line was analyzed, and the differences among each other were determined through Principal Component Analysis according to a protocol previously proposed mainly for electrocardiogram processing and X-ray photoelectron spectroscopy. Our results showed that the four cancer cell lines analyzed exhibited peculiar dielectric properties when compared to each other and the growth medium, confirming that PCA could be employed as an alternative methodology to analyze microwave characterization of cancer cell lines which, in turn, may be deeply exploited as a tool for the detection of cancer cells in healthy tissues.

Author Biographies

Livio D'Alvia, La Sapienza University of Rome, Italy

Research Fellow at the Department of Mechanical and Aerospace Engineering

Serena Carraro, La Sapienza University of Rome, Italy

Ph.D. student at the Department of Mechanical and Aerospace Engineering

Barbara Peruzzi, Bambino Gesù Children's Hospital, Italy

Researcher at the Multifactorial Disease and Complex Phenotype Research Area

Enrica Urciuoli, Bambino Gesù Children's Hospital, Italy

Researcher at the Multifactorial Disease and Complex Phenotype Research Area

Ludovica Apa, La Sapienza University of Rome, Italy

Research Fellow at the Department of Mechanical and Aerospace Engineering

Emanuele Rizzuto, La Sapienza University of Rome, Italy

Assistant Professor at the Department of Mechanical and Aerospace Engineering

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Published

2023-06-09

Issue

Vol. 12 No. 2 (2023)

Section

Research Papers

License

Copyright (c) 2023 Livio D'Alvia, Serena Carraro, Barbara Peruzzi, Enrica Urciuoli, Ludovica Apa, Emanuele Rizzuto

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